Cell Painting, an image-based assay for morphological profiling Authors:
نویسندگان
چکیده
Authors: Mark-Anthony Bray [email protected] Shantanu Singh [email protected] Han Han [email protected] Chadwick T. Davis [email protected] Blake Borgeson [email protected] Cathy Hartland [email protected] Maria Kost-Alimova [email protected] Sigrun M. Gustafsdottir [email protected] Christopher C. Gibson* [email protected] http://www.recursionpharma.com Anne E. Carpenter* [email protected] http://www.broadinstitute.org/~anne/
منابع مشابه
Morphological Profiles of RNAi-Induced Gene Knockdown Are Highly Reproducible but Dominated by Seed Effects
RNA interference and morphological profiling-the measurement of thousands of phenotypes from individual cells by microscopy and image analysis-are a potentially powerful combination. We show that morphological profiles of RNAi-induced knockdown using the Cell Painting assay are in fact highly sensitive and reproducible. However, we find that the magnitude and prevalence of off-target effects vi...
متن کاملSystematic morphological profiling of human gene and allele function via Cell Painting
We hypothesized that human genes and disease-associated alleles might be systematically functionally annotated using morphological profiling of cDNA constructs, via a microscopy-based Cell Painting assay. Indeed, 50% of the 220 tested genes yielded detectable morphological profiles, which grouped into biologically meaningful gene clusters consistent with known functional annotation (e.g., the R...
متن کاملA dataset of images and morphological profiles of 30 000 small-molecule treatments using the Cell Painting assay
Background Large-scale image sets acquired by automated microscopy of perturbed samples enable a detailed comparison of cell states induced by each perturbation, such as a small molecule from a diverse library. Highly multiplexed measurements of cellular morphology can be extracted from each image and subsequently mined for a number of applications. Findings This microscopy dataset includes 9...
متن کاملMultiplex Cytological Profiling Assay to Measure Diverse Cellular States
Computational methods for image-based profiling are under active development, but their success hinges on assays that can capture a wide range of phenotypes. We have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects sev...
متن کاملLC-ESI-MS/MS Alkaloidal Profiling and Biological Investigation of Cleistocactus winteri Stems
Cleistocactus winteri is a succulent plant of the Cactaceae family, commonly known as the golden rat tail. Many members of the Cactaceae family are the focus of chemical and biological studies as they contain bioactive compounds, well known for their health-related properties. We aimed to investigate Cleistocactus winteri stems both phytochemically and biologicallyfor the firs...
متن کامل